TY - JOUR
T1 - C1q as a unique player in angiogenesis with therapeutic implication in wound healing
AU - Bossi, Fleur
AU - Tripodo, Claudio
AU - Rizzi, Lucia
AU - Bulla, Roberta
AU - Agostinis, Chiara
AU - Guarnotta, Carla
AU - Munaut, Carine
AU - Baldassarre, Gustavo
AU - Papa, Giovanni
AU - Zorzet, Sonia
AU - Ghebrehiwet, Berhane
AU - Ling, Guang Sheng
AU - Botto, Marina
AU - Tedesco, Francesco
PY - 2014/3/18
Y1 - 2014/3/18
N2 - We have previously shown that C1q is expressed on endothelial cells (ECs) of newly formed decidual tissue. Here we demonstrate that C1q is deposited in wound-healing skin in the absence of C4 and C3 and that C1q mRNA is locally expressed as revealed by real-time PCR and in situ hybridization. C1q was found to induce permeability of the EC monolayer, to stimulate EC proliferation and migration, and to promote tube formation and sprouting of new vessels in a rat aortic ring assay. Using a murine model of wound healing we observed that vessel formation was defective in C1qa-/- mice and was restored to normal after local application of C1q. The mean vessel density of wound-healing tissue and the healed wound area were significantly increased in C1q-treated rats. On the basis of these results we suggest that C1q may represent a valuable therapeutic agent that can be used to treat chronic ulcers or other pathological conditions in which angiogenesis is impaired, such as myocardial ischemia.
AB - We have previously shown that C1q is expressed on endothelial cells (ECs) of newly formed decidual tissue. Here we demonstrate that C1q is deposited in wound-healing skin in the absence of C4 and C3 and that C1q mRNA is locally expressed as revealed by real-time PCR and in situ hybridization. C1q was found to induce permeability of the EC monolayer, to stimulate EC proliferation and migration, and to promote tube formation and sprouting of new vessels in a rat aortic ring assay. Using a murine model of wound healing we observed that vessel formation was defective in C1qa-/- mice and was restored to normal after local application of C1q. The mean vessel density of wound-healing tissue and the healed wound area were significantly increased in C1q-treated rats. On the basis of these results we suggest that C1q may represent a valuable therapeutic agent that can be used to treat chronic ulcers or other pathological conditions in which angiogenesis is impaired, such as myocardial ischemia.
KW - Animal models
KW - Complement
KW - Vasculogenesis
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U2 - 10.1073/pnas.1311968111
DO - 10.1073/pnas.1311968111
M3 - Article
C2 - 24591625
AN - SCOPUS:84896529758
SN - 0027-8424
VL - 111
SP - 4209
EP - 4214
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 11
ER -