TY - JOUR
T1 - Biosynthesis and physico-chemical characterization of high performing peptide hydrogels@graphene oxide composites
AU - Chronopoulou, Laura
AU - Di Nitto, Antonio
AU - Papi, Massimiliano
AU - Parolini, Ornella
AU - Falconi, Mirella
AU - Teti, Gabriella
AU - Muttini, Aurelio
AU - Lattanzi, Wanda
AU - Palmieri, Valentina
AU - Ciasca, Gabriele
AU - Del Giudice, Alessandra
AU - Galantini, Luciano
AU - Zanoni, Robertino
AU - Palocci, Cleofe
N1 - Funding Information:
The authors acknowledge the SAXSLab facility at Sapienza University. This work benefited from the use of the SasView application, developed under NSF award DMR-0520547. SasView contains codes developed with funding from the EU (Horizon 2020: SINE2020, grant no. 654000 ).
Funding Information:
Ministry of Education, Ministry of University and Research, Italy (MIUR) (grant no. 2017RSAFK7 ).
Publisher Copyright:
© 2021 The Author(s)
PY - 2021/11
Y1 - 2021/11
N2 - Hydrogels based on short peptide molecules are interesting biomaterials with wide present and prospective use in biotechnologies. A well-known possible drawback of these materials can be their limited mechanical performance. In order to overcome this problem, we prepared Fmoc-Phe3self-assembling peptides by a biocatalytic approach, and we reinforced the hydrogel with graphene oxide nanosheets. The formulation here proposed confers to the hydrogel additional physicochemical properties without hampering peptide self-assembly. We investigated in depth the effect of nanocarbon morphology on hydrogel properties (i.e. morphology, viscoelastic properties, stiffness, resistance to an applied stress). In view of further developments towards possible clinical applications, we have preliminarily tested the biocompatibility of the composites. Our results showed that the innovative hydrogel composite formulation based on FmocPhe3 and GO is a biomaterial with improved mechanical properties that appears suitable for the development of biotechnological applications.
AB - Hydrogels based on short peptide molecules are interesting biomaterials with wide present and prospective use in biotechnologies. A well-known possible drawback of these materials can be their limited mechanical performance. In order to overcome this problem, we prepared Fmoc-Phe3self-assembling peptides by a biocatalytic approach, and we reinforced the hydrogel with graphene oxide nanosheets. The formulation here proposed confers to the hydrogel additional physicochemical properties without hampering peptide self-assembly. We investigated in depth the effect of nanocarbon morphology on hydrogel properties (i.e. morphology, viscoelastic properties, stiffness, resistance to an applied stress). In view of further developments towards possible clinical applications, we have preliminarily tested the biocompatibility of the composites. Our results showed that the innovative hydrogel composite formulation based on FmocPhe3 and GO is a biomaterial with improved mechanical properties that appears suitable for the development of biotechnological applications.
KW - Composite
KW - Graphene
KW - Oxide
KW - Peptide hydrogel
KW - Self-assembly
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U2 - 10.1016/j.colsurfb.2021.111989
DO - 10.1016/j.colsurfb.2021.111989
M3 - Article
C2 - 34303114
AN - SCOPUS:85110531904
SN - 0927-7765
VL - 207
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
M1 - 111989
ER -