Apoptosis in HIV infection: Protective role of IL-2

P. Cordiali Fei; M. Solmone; M. Viora; P. Vanacore; O. Pugliese; A. Giglio; F. Caprilli; F. Ameglio

Apoptosis in HIV infection: Protective role of IL-2

P. Cordiali Fei, M. Solmone, M. Viora, P. Vanacore, O. Pugliese, A. Giglio, F. Caprilli, F. Ameglio

Istituto S. Maria e S. Gallicano

Research output: Contribution to journal › Article › peer-review

Abstract

Peripheral blood mononuclear cells from HIV-infected subjects have been demonstrated by different methods to die by apoptosis after short time in culture. In the present study the percentages of apoptotic cells have been measured by propidium iodide staining and flow cytometry in PBMC from healthy controls (15) and HIV-infected subjects with asymptomatic (10) or advanced (15) disease, with or without anti-viral treatment. The percentage of apoptosis significantly correlated with clinical stage (CDCII: 15.85% ± 9.17, CDCIV: 22.6% ± 5.97, P <0.001) and the CD4/CD8 CD3 cell ratio. P = -0.57, P = 0.012), while no differences were found in relation to AZT therapy. By adding IL-2 to the cultures the percentages of apoptosis of PBMC from HIV-infected patients were significantly reduced in all experiments.

Original language	English
Pages (from-to)	60-64
Number of pages	5
Journal	Journal of Biological Regulators and Homeostatic Agents
Volume	8
Issue number	2
Publication status	Published - 1994

Keywords

AIDS
apoptosis
flow cytometry
IL-2

ASJC Scopus subject areas

Agricultural and Biological Sciences(all)
Endocrinology
Physiology
Immunology
Medicine (miscellaneous)
Endocrinology, Diabetes and Metabolism
Physiology (medical)

Cite this

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title = "Apoptosis in HIV infection: Protective role of IL-2",

abstract = "Peripheral blood mononuclear cells from HIV-infected subjects have been demonstrated by different methods to die by apoptosis after short time in culture. In the present study the percentages of apoptotic cells have been measured by propidium iodide staining and flow cytometry in PBMC from healthy controls (15) and HIV-infected subjects with asymptomatic (10) or advanced (15) disease, with or without anti-viral treatment. The percentage of apoptosis significantly correlated with clinical stage (CDCII: 15.85% ± 9.17, CDCIV: 22.6% ± 5.97, P <0.001) and the CD4/CD8 CD3 cell ratio. P = -0.57, P = 0.012), while no differences were found in relation to AZT therapy. By adding IL-2 to the cultures the percentages of apoptosis of PBMC from HIV-infected patients were significantly reduced in all experiments.",

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T2 - Protective role of IL-2

AU - Cordiali Fei, P.

AU - Solmone, M.

AU - Viora, M.

AU - Vanacore, P.

AU - Pugliese, O.

AU - Giglio, A.

AU - Caprilli, F.

AU - Ameglio, F.

PY - 1994

Y1 - 1994

N2 - Peripheral blood mononuclear cells from HIV-infected subjects have been demonstrated by different methods to die by apoptosis after short time in culture. In the present study the percentages of apoptotic cells have been measured by propidium iodide staining and flow cytometry in PBMC from healthy controls (15) and HIV-infected subjects with asymptomatic (10) or advanced (15) disease, with or without anti-viral treatment. The percentage of apoptosis significantly correlated with clinical stage (CDCII: 15.85% ± 9.17, CDCIV: 22.6% ± 5.97, P <0.001) and the CD4/CD8 CD3 cell ratio. P = -0.57, P = 0.012), while no differences were found in relation to AZT therapy. By adding IL-2 to the cultures the percentages of apoptosis of PBMC from HIV-infected patients were significantly reduced in all experiments.

AB - Peripheral blood mononuclear cells from HIV-infected subjects have been demonstrated by different methods to die by apoptosis after short time in culture. In the present study the percentages of apoptotic cells have been measured by propidium iodide staining and flow cytometry in PBMC from healthy controls (15) and HIV-infected subjects with asymptomatic (10) or advanced (15) disease, with or without anti-viral treatment. The percentage of apoptosis significantly correlated with clinical stage (CDCII: 15.85% ± 9.17, CDCIV: 22.6% ± 5.97, P <0.001) and the CD4/CD8 CD3 cell ratio. P = -0.57, P = 0.012), while no differences were found in relation to AZT therapy. By adding IL-2 to the cultures the percentages of apoptosis of PBMC from HIV-infected patients were significantly reduced in all experiments.

KW - AIDS

KW - apoptosis

KW - flow cytometry

KW - IL-2

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Apoptosis in HIV infection: Protective role of IL-2

Abstract

Keywords

ASJC Scopus subject areas

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