Abstract
The aim of this study is to evaluate the effect of clodronate on apoptosis of human systemic lupus erythematosus circulating mononuclear cells and to analyze possible correlations with changes in autoantibody production in vitro. Lympho-monocytes from 20 SLE patients were isolated and incubated with or without addition of 1 μM clodronate for 72 hours. Apoptosis and release of genomic material was assessed by immunofluorescent detection of cleaved caspase-3 and by Cell-Death-Detection ELISAPLUS kit (Roche). Anti-Nucleosome IgG and anti-dsDNA IgM and IgG autoantibody levels were determined in supernatants by commercially available ELISA kits. Clodronate induced apoptosis in monocytes as confirmed by cleaved caspase-3 immunostaining and by quantification of cleaved nucleosome in the supernatants (treated 0.22±0.05 O.D. vs untreated 0.09±0.04 O.D.; P
| Original language | English |
|---|---|
| Pages (from-to) | 535-542 |
| Number of pages | 8 |
| Journal | International Journal of Immunopathology and Pharmacology |
| Volume | 23 |
| Issue number | 2 |
| Publication status | Published - Apr 2010 |
Keywords
- Anti-nucleosome antibodies
- Apoptosis
- Clodronate
- Mononuclear cells
- SLE
ASJC Scopus subject areas
- Pharmacology
- Immunology
- Immunology and Allergy