Antimicrobial Effect and Cytotoxic Evaluation of Mg-Doped Hydroxyapatite Functionalized with Au-Nano Rods

Domenico Franco, Giovanna Calabrese, Salvatore Petralia, Giulia Neri, Carmelo Corsaro, Lucia Forte, Stefano Squarzoni, Salvatore Guglielmino, Francesco Traina, Enza Fazio, Sabrina Conoci

Research output: Contribution to journalArticlepeer-review


Hydroxyapatite (HA) is the main inorganic mineral that constitutes bone matrix and represents the most used biomaterial for bone regeneration. Over the years, it has been demonstrated that HA exhibits good biocompatibility, osteoconductivity, and osteoinductivity both in vitro and in vivo, and can be prepared by synthetic and natural sources via easy fabrication strategies. However, its low antibacterial property and its fragile nature restricts its usage for bone graft applications. In this study we functionalized a MgHA scaffold with gold nanorods (AuNRs) and evaluated its antibacterial effect against S. aureus and E. coli in both suspension and adhesion and its cytotoxicity over time (1 to 24 days). Results show that the AuNRs nano-functionalization improves the antibacterial activity with 100% bacterial reduction after 24 h. The toxicity study, however, indicates a 4.38-fold cell number decrease at 24 days. Although further optimization on nano-functionalization process are needed for cytotoxicity, these data indicated that Au-NRs nano-functionalization is a very promising method for improving the antibacterial properties of HA.

Original languageEnglish
Pages (from-to)1-12
Number of pages12
Issue number4
Publication statusPublished - Feb 19 2021


  • Au nanoparticles
  • antibacterial activity
  • bone tissue engineering
  • cytotoxicity
  • hybrid biomaterials
  • hydroxyapatite
  • nano-functionalization


Dive into the research topics of 'Antimicrobial Effect and Cytotoxic Evaluation of Mg-Doped Hydroxyapatite Functionalized with Au-Nano Rods'. Together they form a unique fingerprint.

Cite this