Angiogenesis as a Novel Component of Inflammatory Bowel Disease Pathogenesis

Silvio Danese, Miquel Sans, Carol de la Motte, Cristina Graziani, Gail West, Manijeh H. Phillips, Roberto Pola, Sergio Rutella, Joe Willis, Antonio Gasbarrini, Claudio Fiocchi

Research output: Contribution to journalArticlepeer-review


Background & Aims: Angiogenesis is a critical component of neoplastic and chronic inflammatory disorders, but whether angiogenesis also occurs in inflammatory bowel disease (IBD) has yet to be established. We assessed mucosal vascularization, expression of endothelial αVβ3 integrin, angiogenic factors, and their bioactivity in Crohn's disease (CD) and ulcerative colitis (UC) mucosa. Methods: Mucosal endothelium was immunostained for CD31 and factor VIII and quantified by digital morphometry. αVβ3 expression was studied in vivo by confocal microscopy and in vitro by flow cytometric analysis of human intestinal microvascular endothelial cells (HIMECs). Vascular endothelial growth factor (VEGF), interleukin (IL)-8, and bFGF levels were measured in mucosal extracts and cells and angiogenic bioactivity shown by induction of HIMEC migration and the corneal and chorioallantoic membrane angiogenesis assays. Results: Microvessel density was increased in IBD mucosa. Endothelial αVβ3 was strongly expressed in IBD but only sporadically in normal mucosa and was up-regulated in HIMECs by VEGF, tumor necrosis factor α, and bFGF. IBD mucosal extracts induced a significantly higher degree of HIMEC migration than control mucosa, and this response was mostly dependent on IL-8 and less on basic fibroblast growth factor or vascular endothelial growth factor. Compared with normal mucosa, IBD mucosal extracts induced a potent angiogenic response in both the corneal and chorioallantoic membrane assays. Conclusions: These results provide morphological, phenotypic and functional evidence of potent angiogenic activity in both CD and UC mucosa, indicating that the local microvasculature undergoes an intense process of inflammation-dependent angiogenesis. Thus, angiogenesis appears to be an integral component of IBD pathogenesis, providing the practical and conceptual framework for anti-angiogenic therapies in IBD.

Original languageEnglish
Pages (from-to)2060-2073
Number of pages14
Issue number7
Publication statusPublished - Jul 2006

ASJC Scopus subject areas

  • Gastroenterology


Dive into the research topics of 'Angiogenesis as a Novel Component of Inflammatory Bowel Disease Pathogenesis'. Together they form a unique fingerprint.

Cite this