TY - JOUR
T1 - An original application of plasma expanders
T2 - Heart-lung preservation
AU - Spaggiari, L.
AU - Rusca, M.
AU - Carbognani, P.
AU - Alfieri, R.
AU - Urbani, S.
AU - Petronini, P. G.
AU - Cattelani, L.
AU - Borghetti, A. F.
AU - Bobbio, P.
PY - 1996/12
Y1 - 1996/12
N2 - The lack of an ideal heart-lung preservation solution is one of the principal factor that limits the wide spread of transplantation. The aim of this work was to investigate the efficacy of Haemaccel (HM) on isolated human pulmonary artery endothelial cells comparing its effects with those of University of Wisconsin (UWS). Subcultures of HPAEC were inoculated at the density of 5,000 cells per cm2 in 9 cm2 well-plates. Cells were incubated with HM and UWS for 6 hrs at 10°C. Cellular viability was analysed by the total protein content (cytotoxicity index) and by the rate of protein synthesis (metabolic index). The results showed that HM and UWS did no show a significant differences in the toxicity when compared with the control; on the contrary, HM seems to determine a less inhibitory effect on cellular metabolism permitting a more rapid cellular metabolic recovery than UWS. Thus, HM appears to be more suitable for the preservation of isolated HPAEC than UWS.
AB - The lack of an ideal heart-lung preservation solution is one of the principal factor that limits the wide spread of transplantation. The aim of this work was to investigate the efficacy of Haemaccel (HM) on isolated human pulmonary artery endothelial cells comparing its effects with those of University of Wisconsin (UWS). Subcultures of HPAEC were inoculated at the density of 5,000 cells per cm2 in 9 cm2 well-plates. Cells were incubated with HM and UWS for 6 hrs at 10°C. Cellular viability was analysed by the total protein content (cytotoxicity index) and by the rate of protein synthesis (metabolic index). The results showed that HM and UWS did no show a significant differences in the toxicity when compared with the control; on the contrary, HM seems to determine a less inhibitory effect on cellular metabolism permitting a more rapid cellular metabolic recovery than UWS. Thus, HM appears to be more suitable for the preservation of isolated HPAEC than UWS.
KW - Heart-lung preservation
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UR - http://www.scopus.com/inward/citedby.url?scp=0030308182&partnerID=8YFLogxK
M3 - Article
C2 - 10064361
AN - SCOPUS:0030308182
SN - 0021-9509
VL - 37
SP - 109
EP - 112
JO - Journal of Cardiovascular Surgery
JF - Journal of Cardiovascular Surgery
IS - 6 SUPPL. 1
ER -