Adhesion molecules expression in noncrescentic acute post-streptococcal glomerulonephritis

Maria Pia Rastaldi, Franco Ferrario, Lin Yang, Stefania Tunesi, Antonio Indaco, Hequn Zou, Giuseppe D'Amico

Research output: Contribution to journalArticlepeer-review

Abstract

Clinicomorphological features of 11 cases of non-crescentic acute post- streptococcal glomerulonephritis (APSGN) were reviewed. Intraglomerular and interstitial leukocytes and their possible correlation with the adhesion molecules intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and endothelial-leukocyte adhesion molecule-1 (ELAM- 1/E-selectin) were investigated by an immunohistochemical method. Intraglomerular leukocytes were primarily granulocytes (11.4 ± 10 cells/glomerular cross-section) and monocytes-macrophages (13.4 ± 19.4 cells/glomerular cross-section). The granulocytes outnumbered monocytes- macrophages in 7 of 11 specimens. The number of intraglomerular leukocytes correlated with protein-uria at the time of renal biopsy. Intraglomerular ICAM-1 staining was strongly positive in all biopsies, especially when intraglomerular monocytes-macrophages prevailed. Expression of intraglomerular VCAM-1 and E-selectin in diseased kidneys did not differ from that in normal kidneys. Interstitial leukocytes were primarily monocytes- macrophages (158.9 ± 96.8 cells/mm2) and T lymphocytes (102.2 ± 63.9 cells/mm2). The number of interstitial leukocytes, especially monocytes- macrophages, correlated with serum creatinine level at the time of biopsy. Interstitial ICAM-1 staining was strongly positive on tubules, peritubular capillaries, and small vessels. The tubular positivity for ICAM-1 correlated with the number of interstitial monocytes-macrophages. Interstitial VCAM-1 and E-selectin were expressed as in normal kidney tissues. The data from this study demonstrate that APSGN is characterized by the presence of both intraglomerular and interstitial leukocyte infiltration, correlating respectively with proteinuria and serum creatinine at the time of renal biopsy. Among the adhesion molecules studied, ICAM-1 seems the most involved in leukocyte recruitment, especially in that of monocytes-macrophages.

Original languageEnglish
Pages (from-to)2419-2427
Number of pages9
JournalJournal of the American Society of Nephrology
Volume7
Issue number11
Publication statusPublished - Nov 1996

Keywords

  • Acute post- streptococcal glomerulonephritis
  • E-selectin
  • ICAM-1
  • Leukocyte infiltration
  • VCAM-1

ASJC Scopus subject areas

  • Nephrology

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