TY - JOUR
T1 - Activation of mammalian target of rapamycin (mTOR) in triple negative feline mammary carcinomas
AU - Maniscalco, Lorella
AU - Millán, Yolanda
AU - Iussich, Selina
AU - Denina, Mauro
AU - Sánchez-Céspedes, Raquel
AU - Gattino, Francesca
AU - Biolatti, Bartolomeo
AU - Sasaki, Nobuo
AU - Nakagawa, Takayuki
AU - Di Renzo, Maria Flavia
AU - de las Mulas, Juana Martín
AU - De Maria, Raffaella
PY - 2013/4/15
Y1 - 2013/4/15
N2 - Background: Triple negative breast cancer (TNBC) in humans is defined by the absence of oestrogen receptor (ER), progesterone receptor (PR) and HER2 overexpression. Mammalian target of rapamycin (mTOR) is overexpressed in TNBC and it represents a potential target for the treatment of this aggressive tumour. Feline mammary carcinoma (FMC) is considered to be a model for hormone-independent human breast cancer. This study investigated mTOR and p-mTOR expression in FMC in relation to triple negative (TN) phenotype.Results: The expression of mTOR, p-mTOR, ERα, PR and HER2 was evaluated in 58 FMCs by immunohistochemistry and in six FMC cell lines by Western blot analysis. 53.5% of FMC analyzed were ER, PR, HER2 negative (TN-FMC) while 56.9% and 55.2% of cases expressed mTOR and p-mTOR respectively. In this study we found that m-TOR and p-mTOR were more frequently detected in TN-FMC and in HER2 negative samples.Conclusions: In this study, we demonstrate that there is also a FMC subset defined as TN FMC, which is characterised by a statistically significant association with m-TOR and p-mTOR expression as demonstrated in human breast cancer.
AB - Background: Triple negative breast cancer (TNBC) in humans is defined by the absence of oestrogen receptor (ER), progesterone receptor (PR) and HER2 overexpression. Mammalian target of rapamycin (mTOR) is overexpressed in TNBC and it represents a potential target for the treatment of this aggressive tumour. Feline mammary carcinoma (FMC) is considered to be a model for hormone-independent human breast cancer. This study investigated mTOR and p-mTOR expression in FMC in relation to triple negative (TN) phenotype.Results: The expression of mTOR, p-mTOR, ERα, PR and HER2 was evaluated in 58 FMCs by immunohistochemistry and in six FMC cell lines by Western blot analysis. 53.5% of FMC analyzed were ER, PR, HER2 negative (TN-FMC) while 56.9% and 55.2% of cases expressed mTOR and p-mTOR respectively. In this study we found that m-TOR and p-mTOR were more frequently detected in TN-FMC and in HER2 negative samples.Conclusions: In this study, we demonstrate that there is also a FMC subset defined as TN FMC, which is characterised by a statistically significant association with m-TOR and p-mTOR expression as demonstrated in human breast cancer.
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U2 - 10.1186/1746-6148-9-80
DO - 10.1186/1746-6148-9-80
M3 - Article
C2 - 23587222
AN - SCOPUS:84876127232
SN - 1746-6148
VL - 9
JO - BMC Veterinary Research
JF - BMC Veterinary Research
M1 - 80
ER -