Aberrant genes promoter methylation in neural crest-derived tumors

Annamaria La Torre; Lucia Anna Muscarella; Paola Parrella; Teresa Balsamo; Michele Bisceglia; Vanna Maria Valori; Antonella La Torre; Raffaela Barbano; Eleonora Perrella; Maria Luana Poeta; Gennaro Melchionda; Giuseppe Merla; Evaristo Maiello; Riccardo Pellicano; Vito Michele Fazio

doi:10.5301/JBM.2012.9766

Aberrant genes promoter methylation in neural crest-derived tumors

Annamaria La Torre, Lucia Anna Muscarella, Paola Parrella, Teresa Balsamo, Michele Bisceglia, Vanna Maria Valori, Antonella La Torre, Raffaela Barbano, Eleonora Perrella, Maria Luana Poeta, Gennaro Melchionda, Giuseppe Merla, Evaristo Maiello, Riccardo Pellicano, Vito Michele Fazio

IRCCS Casa Sollievo della Sofferenza

Research output: Contribution to journal › Article › peer-review

Abstract

Disturbances in the epigenetic landscape by aberrant methylation of CpG islands can lead to inactivation of cancer-related genes in solid tumors. We analyzed the promoter methylation status of 6 genes previously reported as cancer-specific methylated (MCAM, SSBP2, NISCH, B4GALT1, KIF1A and RASSF1A) in 38 neural crest-derived tumors by quantitative methylation- specific real-time PCR (QMSP). The results demonstrated that the determination of the methylation status of RASSF1A is able to distinguish between normal and tumor samples in cutaneous melanomas, lung carcinoids and small bowel carcinoids. MCAM methylation levels were significantly higher in lung carcinoids tumors (p=0.001), suggesting that this alteration may represent a molecular biomarker in this tumor type.

Original language	English
Journal	International Journal of Biological Markers
Volume	27
Issue number	4
DOIs	https://doi.org/10.5301/JBM.2012.9766
Publication status	Published - Oct 2012

Keywords

Mcam
Methylation
Neural crest-derived tumors
Rassf1a

ASJC Scopus subject areas

Clinical Biochemistry
Cancer Research
Oncology
Pathology and Forensic Medicine

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10.5301/JBM.2012.9766

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La Torre, A., Muscarella, L. A., Parrella, P., Balsamo, T., Bisceglia, M., Valori, V. M., La Torre, A., Barbano, R., Perrella, E., Poeta, M. L., Melchionda, G., Merla, G., Maiello, E., Pellicano, R., & Fazio, V. M. (2012). Aberrant genes promoter methylation in neural crest-derived tumors. International Journal of Biological Markers, 27(4). https://doi.org/10.5301/JBM.2012.9766

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title = "Aberrant genes promoter methylation in neural crest-derived tumors",

abstract = "Disturbances in the epigenetic landscape by aberrant methylation of CpG islands can lead to inactivation of cancer-related genes in solid tumors. We analyzed the promoter methylation status of 6 genes previously reported as cancer-specific methylated (MCAM, SSBP2, NISCH, B4GALT1, KIF1A and RASSF1A) in 38 neural crest-derived tumors by quantitative methylation- specific real-time PCR (QMSP). The results demonstrated that the determination of the methylation status of RASSF1A is able to distinguish between normal and tumor samples in cutaneous melanomas, lung carcinoids and small bowel carcinoids. MCAM methylation levels were significantly higher in lung carcinoids tumors (p=0.001), suggesting that this alteration may represent a molecular biomarker in this tumor type.",

keywords = "Mcam, Methylation, Neural crest-derived tumors, Rassf1a",

author = "{La Torre}, Annamaria and Muscarella, {Lucia Anna} and Paola Parrella and Teresa Balsamo and Michele Bisceglia and Valori, {Vanna Maria} and {La Torre}, Antonella and Raffaela Barbano and Eleonora Perrella and Poeta, {Maria Luana} and Gennaro Melchionda and Giuseppe Merla and Evaristo Maiello and Riccardo Pellicano and Fazio, {Vito Michele}",

year = "2012",

month = oct,

doi = "10.5301/JBM.2012.9766",

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AU - La Torre, Annamaria

AU - Muscarella, Lucia Anna

AU - Parrella, Paola

AU - Balsamo, Teresa

AU - Bisceglia, Michele

AU - Valori, Vanna Maria

AU - La Torre, Antonella

AU - Barbano, Raffaela

AU - Perrella, Eleonora

AU - Poeta, Maria Luana

AU - Melchionda, Gennaro

AU - Merla, Giuseppe

AU - Maiello, Evaristo

AU - Pellicano, Riccardo

AU - Fazio, Vito Michele

PY - 2012/10

Y1 - 2012/10

N2 - Disturbances in the epigenetic landscape by aberrant methylation of CpG islands can lead to inactivation of cancer-related genes in solid tumors. We analyzed the promoter methylation status of 6 genes previously reported as cancer-specific methylated (MCAM, SSBP2, NISCH, B4GALT1, KIF1A and RASSF1A) in 38 neural crest-derived tumors by quantitative methylation- specific real-time PCR (QMSP). The results demonstrated that the determination of the methylation status of RASSF1A is able to distinguish between normal and tumor samples in cutaneous melanomas, lung carcinoids and small bowel carcinoids. MCAM methylation levels were significantly higher in lung carcinoids tumors (p=0.001), suggesting that this alteration may represent a molecular biomarker in this tumor type.

AB - Disturbances in the epigenetic landscape by aberrant methylation of CpG islands can lead to inactivation of cancer-related genes in solid tumors. We analyzed the promoter methylation status of 6 genes previously reported as cancer-specific methylated (MCAM, SSBP2, NISCH, B4GALT1, KIF1A and RASSF1A) in 38 neural crest-derived tumors by quantitative methylation- specific real-time PCR (QMSP). The results demonstrated that the determination of the methylation status of RASSF1A is able to distinguish between normal and tumor samples in cutaneous melanomas, lung carcinoids and small bowel carcinoids. MCAM methylation levels were significantly higher in lung carcinoids tumors (p=0.001), suggesting that this alteration may represent a molecular biomarker in this tumor type.

KW - Mcam

KW - Methylation

KW - Neural crest-derived tumors

KW - Rassf1a

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Aberrant genes promoter methylation in neural crest-derived tumors

Abstract

Keywords

ASJC Scopus subject areas

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