A specific gas chromatographic method for the determination of microsomal styrene monooxygenase and styrene epoxide hydratase activities

G. Belvedere; J. Pachecka; L. Cantoni; E. Mussini; M. Salmona

doi:10.1016/S0021-9673(00)82176-7

A specific gas chromatographic method for the determination of microsomal styrene monooxygenase and styrene epoxide hydratase activities

G. Belvedere, J. Pachecka, L. Cantoni, E. Mussini, M. Salmona

Istituto di Ricerche Farmacologiche "Mario Negri"

Research output: Contribution to journal › Article › peer-review

Abstract

A gas chromatographic (GC) method for the determination of the metabolite resulting from the activities of microsomal styrene monooxygenase (epoxide synthetase) and epoxide hydratase using styrene or styrene epoxide as substrates has been developed. The determination of the activities of both enzymes is based on the GC determination of phenylethylene glycol after its esterification with n-butylboronic acid. Kinetic parameters for both enzymes are given.

Original language	English
Pages (from-to)	387-393
Number of pages	7
Journal	Journal of Chromatography A
Volume	118
Issue number	3
DOIs	https://doi.org/10.1016/S0021-9673(00)82176-7
Publication status	Published - Apr 7 1976

ASJC Scopus subject areas

Analytical Chemistry

Access to Document

10.1016/S0021-9673(00)82176-7

Cite this

@article{1d396dec765f4bb0b37dc62d0a6d7f73,

title = "A specific gas chromatographic method for the determination of microsomal styrene monooxygenase and styrene epoxide hydratase activities",

abstract = "A gas chromatographic (GC) method for the determination of the metabolite resulting from the activities of microsomal styrene monooxygenase (epoxide synthetase) and epoxide hydratase using styrene or styrene epoxide as substrates has been developed. The determination of the activities of both enzymes is based on the GC determination of phenylethylene glycol after its esterification with n-butylboronic acid. Kinetic parameters for both enzymes are given.",

author = "G. Belvedere and J. Pachecka and L. Cantoni and E. Mussini and M. Salmona",

year = "1976",

month = apr,

day = "7",

doi = "10.1016/S0021-9673(00)82176-7",

language = "English",

volume = "118",

pages = "387--393",

journal = "Journal of Chromatography A",

issn = "0021-9673",

publisher = "Elsevier",

number = "3",

}

TY - JOUR

T1 - A specific gas chromatographic method for the determination of microsomal styrene monooxygenase and styrene epoxide hydratase activities

AU - Belvedere, G.

AU - Pachecka, J.

AU - Cantoni, L.

AU - Mussini, E.

AU - Salmona, M.

PY - 1976/4/7

Y1 - 1976/4/7

N2 - A gas chromatographic (GC) method for the determination of the metabolite resulting from the activities of microsomal styrene monooxygenase (epoxide synthetase) and epoxide hydratase using styrene or styrene epoxide as substrates has been developed. The determination of the activities of both enzymes is based on the GC determination of phenylethylene glycol after its esterification with n-butylboronic acid. Kinetic parameters for both enzymes are given.

AB - A gas chromatographic (GC) method for the determination of the metabolite resulting from the activities of microsomal styrene monooxygenase (epoxide synthetase) and epoxide hydratase using styrene or styrene epoxide as substrates has been developed. The determination of the activities of both enzymes is based on the GC determination of phenylethylene glycol after its esterification with n-butylboronic acid. Kinetic parameters for both enzymes are given.

UR - http://www.scopus.com/inward/record.url?scp=0017300511&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017300511&partnerID=8YFLogxK

U2 - 10.1016/S0021-9673(00)82176-7

DO - 10.1016/S0021-9673(00)82176-7

M3 - Article

C2 - 1254672

AN - SCOPUS:0017300511

SN - 0021-9673

VL - 118

SP - 387

EP - 393

JO - Journal of Chromatography A

JF - Journal of Chromatography A

IS - 3

ER -

A specific gas chromatographic method for the determination of microsomal styrene monooxygenase and styrene epoxide hydratase activities

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this