A rapid qPCR method to investigate the circulation of the yeast Wickerhamomyces anomalus in humans

Sara Epis; Aida Capone; Elena Martin; Michela Paolucci; Chiara Bazzocchi; Matteo Valzano; Jovana Bozic; Stefano Novati; Guido Favia; Irene Ricci

A rapid qPCR method to investigate the circulation of the yeast Wickerhamomyces anomalus in humans

Sara Epis, Aida Capone, Elena Martin, Michela Paolucci, Chiara Bazzocchi, Matteo Valzano, Jovana Bozic, Stefano Novati, Guido Favia, Irene Ricci

IRCCS Fondazione Policlinico San Matteo

Research output: Contribution to journal › Article › peer-review

Abstract

The yeast Wickerhamomyces anomalus has been proposed for many biotechnological applications in the food industry. However, a number of opportunistic pathogenic strains have been reported as causative agents of nosocomial fungemia. Recognition of potentially pathogenic isolates is an important challenge for the future commercialization of this yeast. The isolation of W. anomalus from different matrices and, recently, from mosquitoes, requires further investigations into its circulation in humans. Here we present a qPCR protocol for the detection of W. anomalus in human blood samples and the results of a screening of 525 donors, including different classes of patients and healthy people.

Original language	English
Pages (from-to)	577-581
Number of pages	5
Journal	New Microbiologica
Volume	38
Issue number	4
Publication status	Published - Oct 1 2015

Keywords

Fungemia
Mosquito vectors
Opportunistic pathogen
QPCR
Wickerhamomyces anomalus

ASJC Scopus subject areas

Microbiology (medical)

Cite this

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AU - Martin, Elena

AU - Paolucci, Michela

AU - Bazzocchi, Chiara

AU - Valzano, Matteo

AU - Bozic, Jovana

AU - Novati, Stefano

AU - Favia, Guido

AU - Ricci, Irene

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AB - The yeast Wickerhamomyces anomalus has been proposed for many biotechnological applications in the food industry. However, a number of opportunistic pathogenic strains have been reported as causative agents of nosocomial fungemia. Recognition of potentially pathogenic isolates is an important challenge for the future commercialization of this yeast. The isolation of W. anomalus from different matrices and, recently, from mosquitoes, requires further investigations into its circulation in humans. Here we present a qPCR protocol for the detection of W. anomalus in human blood samples and the results of a screening of 525 donors, including different classes of patients and healthy people.

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A rapid qPCR method to investigate the circulation of the yeast Wickerhamomyces anomalus in humans

Abstract

Keywords

ASJC Scopus subject areas

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