A rapid and robust UHPLC-DAD method for the quantification of amphotericin B in human plasma

Sebastiano Barco; Alessia Zunino; Antonio D'Avolio; Laura Barbagallo; Angelo Maffia; Gino Tripodi; Elio Castagnola; Giuliana Cangemi

doi:10.1016/j.jpba.2017.01.048

A rapid and robust UHPLC-DAD method for the quantification of amphotericin B in human plasma

Sebastiano Barco, Alessia Zunino, Antonio D'Avolio, Laura Barbagallo, Angelo Maffia, Gino Tripodi, Elio Castagnola, Giuliana Cangemi

Istituto "Giannina Gaslini"

Research output: Contribution to journal › Article › peer-review

Abstract

Amphotericin B is an antifungal drug widely used in Intensive Care Units. Therapeutic drug monitoring (TDM) of amphotericin B is recommended for the assessment of toxicity surveillance and treatment optimization. In this paper we described the development and validation of a new Ultra High Performance Liquid Chromatography coupled to Diode Array Detection (UHPLC-DAD) method for the quantification of Amphotericin B in 200μL human plasma over a wide range of concentrations (0.125-10mg/L). The new method has been validated following international guidelines on bioanalytical method validation and showed high selectivity, high accuracy and precision and high process efficiency. The new UHPLC-DAD method that we describe is robust, rapid, cost effective and suitable for application to the routine TDM analyses.

Original language	English
Pages (from-to)	142-145
Number of pages	4
Journal	Journal of Pharmaceutical and Biomedical Analysis
Volume	138
DOIs	https://doi.org/10.1016/j.jpba.2017.01.048
Publication status	Published - May 10 2017

Keywords

Adolescent
Amphotericin B
Antifungal Agents
Child
Child, Preschool
Chromatography, High Pressure Liquid
Drug Monitoring
Female
Humans
Infant
Male
Plasma
Reproducibility of Results
Journal Article

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10.1016/j.jpba.2017.01.048

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title = "A rapid and robust UHPLC-DAD method for the quantification of amphotericin B in human plasma",

abstract = "Amphotericin B is an antifungal drug widely used in Intensive Care Units. Therapeutic drug monitoring (TDM) of amphotericin B is recommended for the assessment of toxicity surveillance and treatment optimization. In this paper we described the development and validation of a new Ultra High Performance Liquid Chromatography coupled to Diode Array Detection (UHPLC-DAD) method for the quantification of Amphotericin B in 200μL human plasma over a wide range of concentrations (0.125-10mg/L). The new method has been validated following international guidelines on bioanalytical method validation and showed high selectivity, high accuracy and precision and high process efficiency. The new UHPLC-DAD method that we describe is robust, rapid, cost effective and suitable for application to the routine TDM analyses.",

keywords = "Adolescent, Amphotericin B, Antifungal Agents, Child, Child, Preschool, Chromatography, High Pressure Liquid, Drug Monitoring, Female, Humans, Infant, Male, Plasma, Reproducibility of Results, Journal Article",

author = "Sebastiano Barco and Alessia Zunino and Antonio D'Avolio and Laura Barbagallo and Angelo Maffia and Gino Tripodi and Elio Castagnola and Giuliana Cangemi",

year = "2017",

month = may,

day = "10",

doi = "10.1016/j.jpba.2017.01.048",

language = "English",

volume = "138",

pages = "142--145",

journal = "Journal of Pharmaceutical and Biomedical Analysis",

issn = "0731-7085",

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T1 - A rapid and robust UHPLC-DAD method for the quantification of amphotericin B in human plasma

AU - Barco, Sebastiano

AU - Zunino, Alessia

AU - D'Avolio, Antonio

AU - Barbagallo, Laura

AU - Maffia, Angelo

AU - Tripodi, Gino

AU - Castagnola, Elio

AU - Cangemi, Giuliana

PY - 2017/5/10

Y1 - 2017/5/10

N2 - Amphotericin B is an antifungal drug widely used in Intensive Care Units. Therapeutic drug monitoring (TDM) of amphotericin B is recommended for the assessment of toxicity surveillance and treatment optimization. In this paper we described the development and validation of a new Ultra High Performance Liquid Chromatography coupled to Diode Array Detection (UHPLC-DAD) method for the quantification of Amphotericin B in 200μL human plasma over a wide range of concentrations (0.125-10mg/L). The new method has been validated following international guidelines on bioanalytical method validation and showed high selectivity, high accuracy and precision and high process efficiency. The new UHPLC-DAD method that we describe is robust, rapid, cost effective and suitable for application to the routine TDM analyses.

AB - Amphotericin B is an antifungal drug widely used in Intensive Care Units. Therapeutic drug monitoring (TDM) of amphotericin B is recommended for the assessment of toxicity surveillance and treatment optimization. In this paper we described the development and validation of a new Ultra High Performance Liquid Chromatography coupled to Diode Array Detection (UHPLC-DAD) method for the quantification of Amphotericin B in 200μL human plasma over a wide range of concentrations (0.125-10mg/L). The new method has been validated following international guidelines on bioanalytical method validation and showed high selectivity, high accuracy and precision and high process efficiency. The new UHPLC-DAD method that we describe is robust, rapid, cost effective and suitable for application to the routine TDM analyses.

KW - Adolescent

KW - Amphotericin B

KW - Antifungal Agents

KW - Child

KW - Child, Preschool

KW - Chromatography, High Pressure Liquid

KW - Drug Monitoring

KW - Female

KW - Humans

KW - Infant

KW - Male

KW - Plasma

KW - Reproducibility of Results

KW - Journal Article

U2 - 10.1016/j.jpba.2017.01.048

DO - 10.1016/j.jpba.2017.01.048

M3 - Article

C2 - 28199895

SN - 0731-7085

VL - 138

SP - 142

EP - 145

JO - Journal of Pharmaceutical and Biomedical Analysis

JF - Journal of Pharmaceutical and Biomedical Analysis

ER -

A rapid and robust UHPLC-DAD method for the quantification of amphotericin B in human plasma

Abstract

Keywords

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