A rapid and quantitative mass spectrometry method for determining the concentration of acylcarnitines and aminoacids in amniotic fluid

Laura Braida; Sergio Crovella; Michele Boniotto; Ariella Luchesi; Umberto De Vonderweid; Bruno Casetta; Antonio Amoroso

doi:10.1002/pd.93

A rapid and quantitative mass spectrometry method for determining the concentration of acylcarnitines and aminoacids in amniotic fluid

Laura Braida, Sergio Crovella, Michele Boniotto, Ariella Luchesi, Umberto De Vonderweid, Bruno Casetta, Antonio Amoroso

IRCCS pediatrico Burlo Garofolo

Research output: Contribution to journal › Article › peer-review

Abstract

We describe a quantitative, rapid, sensitive and reproducible tandem mass spectrometry (MSMS) method for the one-step detection of aminoacid (AAs) and acylcarnitine (ACs) concentrations in amniotic fluid. This technology is quicker and more sensitive than other methods used to date since it is possible to determine very low AA and AC concentrations in samples simultaneously in a single run. The high degree of automation allows a large number of pregnancies to be screened for metabolic defects in a very short time.

Original language	English
Pages (from-to)	543-546
Number of pages	4
Journal	Prenatal Diagnosis
Volume	21
Issue number	7
DOIs	https://doi.org/10.1002/pd.93
Publication status	Published - 2001

Keywords

Acylcarnitines
Aminoacids
Amniotic fluid
Mass spectrometry

ASJC Scopus subject areas

Genetics(clinical)
Obstetrics and Gynaecology

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10.1002/pd.93

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abstract = "We describe a quantitative, rapid, sensitive and reproducible tandem mass spectrometry (MSMS) method for the one-step detection of aminoacid (AAs) and acylcarnitine (ACs) concentrations in amniotic fluid. This technology is quicker and more sensitive than other methods used to date since it is possible to determine very low AA and AC concentrations in samples simultaneously in a single run. The high degree of automation allows a large number of pregnancies to be screened for metabolic defects in a very short time.",

keywords = "Acylcarnitines, Aminoacids, Amniotic fluid, Mass spectrometry",

author = "Laura Braida and Sergio Crovella and Michele Boniotto and Ariella Luchesi and {De Vonderweid}, Umberto and Bruno Casetta and Antonio Amoroso",

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AU - Braida, Laura

AU - Crovella, Sergio

AU - Boniotto, Michele

AU - Luchesi, Ariella

AU - De Vonderweid, Umberto

AU - Casetta, Bruno

AU - Amoroso, Antonio

PY - 2001

Y1 - 2001

N2 - We describe a quantitative, rapid, sensitive and reproducible tandem mass spectrometry (MSMS) method for the one-step detection of aminoacid (AAs) and acylcarnitine (ACs) concentrations in amniotic fluid. This technology is quicker and more sensitive than other methods used to date since it is possible to determine very low AA and AC concentrations in samples simultaneously in a single run. The high degree of automation allows a large number of pregnancies to be screened for metabolic defects in a very short time.

AB - We describe a quantitative, rapid, sensitive and reproducible tandem mass spectrometry (MSMS) method for the one-step detection of aminoacid (AAs) and acylcarnitine (ACs) concentrations in amniotic fluid. This technology is quicker and more sensitive than other methods used to date since it is possible to determine very low AA and AC concentrations in samples simultaneously in a single run. The high degree of automation allows a large number of pregnancies to be screened for metabolic defects in a very short time.

KW - Acylcarnitines

KW - Aminoacids

KW - Amniotic fluid

KW - Mass spectrometry

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A rapid and quantitative mass spectrometry method for determining the concentration of acylcarnitines and aminoacids in amniotic fluid

Abstract

Keywords

ASJC Scopus subject areas

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