A Novel Vasoactive Peptide "PG1" from Buffalo Ice-Cream Protects from Angiotensin-Evoked High Blood Pressure

Albino Carrizzo, Manuela Giovanna Basilicata, Giacomo Pepe, Kasper K Sørensen, Michele Ciccarelli, Veronica Di Sarno, Antonio Damato, Eleonora Venturini, Anna Borrelli, Simona Musella, Mario Abate, Paola Di Pietro, Carmine Ostacolo, Pietro Campiglia, Carmine Vecchione

Research output: Contribution to journalArticlepeer-review


BACKGROUND: Arterial hypertension is the most important risk factor for cardiovascular diseases, myocardial infarction, heart failure, renal failure and peripheral vascular disease. In the last decade, milk-derived bioactive peptides have attracted attention for their beneficial cardiovascular properties.

METHODS: Here, we combined in vitro chemical assay such as LC-MS/MS analysis of buffalo ice cream, ex vivo vascular studies evaluating endothelial and smooth muscle responses using pressure myograph, and translational assay testing in vivo the vascular actions of PG1 administration in murine models.

RESULTS: We demonstrate that a novel buffalo ice-cream-derived pentapeptide "QKEPM", namely PG1, is a stable peptide that can be obtained at higher concentration after gastro-intestinal digestions (GID) of buffalo ice-cream (BIC). It owns potent vascular effect in counteract the effects of angiotensin II-evoked vasoconstriction and high blood pressure levels. Its effects are mediated by the inhibitory effect on AT1 receptor leading to a downregulation of p-ERK½/Rac1-GTP and consequent reduction of oxidative stress.

CONCLUSIONS: These results strongly candidate PG1, as a novel bioactive peptide for the prevention and management of hypertension, thus expanding the armamentarium of preventive strategies aimed at reducing the incidence and progression of hypertension and its related cardiovascular complications.

Original languageEnglish
Issue number3
Publication statusPublished - Mar 12 2021


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