A newborn rat model for the study of cerebral hemodynamics by near-infrared spectroscopy and laser-Doppler flowmetry in the immature brain

Monica Fumagalli; Fabio Mosca; Gitte Moos Knudsen; Gorm Greisen

doi:10.1159/000074967

A newborn rat model for the study of cerebral hemodynamics by near-infrared spectroscopy and laser-Doppler flowmetry in the immature brain

Monica Fumagalli, Fabio Mosca, Gitte Moos Knudsen, Gorm Greisen

Fondazione IRCCS Ca' Granda- Ospedale Maggiore Policlinico

Research output: Contribution to journal › Article › peer-review

Abstract

An animal model for the study of cerebrovascular physiology in the immature brain was developed. Twelve 3- to 5-day-old rat pups were maintained on spontaneous breathing under light anesthesia for either 1 or 2 h. Transcutaneous carbon dioxide tension and arterial oxygen saturation were monitored. Continuous infusion of doxapram limited respiratory acidosis. Cerebral blood flow (CBF) and volume (CBV) could be monitored by near-infrared spectroscopy (NIRS) and laser-Doppler flowmetry (LDF) in spite of some movement artifacts. CBV and CBF were 6.0 ± 0.3 SE ml/100 g and 36.3 ± 3.1 SE ml/100 g/min, respectively, and remained stable during the study. Cerebrovascular responses, as monitored by LDF and NIRS, to hypoxic and hypercapnic gas mixtures were consistent.

Original language	English
Pages (from-to)	112-120
Number of pages	9
Journal	Biology of the Neonate
Volume	85
Issue number	2
DOIs	https://doi.org/10.1159/000074967
Publication status	Published - 2004

Keywords

Cerebrovascular reactivity
Hypercapnia
Hypoxia
Laser-Doppler flowmetry
Near-infrared spectroscopy
Newborn rat

ASJC Scopus subject areas

Developmental Biology
Pediatrics, Perinatology, and Child Health

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10.1159/000074967

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title = "A newborn rat model for the study of cerebral hemodynamics by near-infrared spectroscopy and laser-Doppler flowmetry in the immature brain",

abstract = "An animal model for the study of cerebrovascular physiology in the immature brain was developed. Twelve 3- to 5-day-old rat pups were maintained on spontaneous breathing under light anesthesia for either 1 or 2 h. Transcutaneous carbon dioxide tension and arterial oxygen saturation were monitored. Continuous infusion of doxapram limited respiratory acidosis. Cerebral blood flow (CBF) and volume (CBV) could be monitored by near-infrared spectroscopy (NIRS) and laser-Doppler flowmetry (LDF) in spite of some movement artifacts. CBV and CBF were 6.0 ± 0.3 SE ml/100 g and 36.3 ± 3.1 SE ml/100 g/min, respectively, and remained stable during the study. Cerebrovascular responses, as monitored by LDF and NIRS, to hypoxic and hypercapnic gas mixtures were consistent.",

keywords = "Cerebrovascular reactivity, Hypercapnia, Hypoxia, Laser-Doppler flowmetry, Near-infrared spectroscopy, Newborn rat",

author = "Monica Fumagalli and Fabio Mosca and Knudsen, {Gitte Moos} and Gorm Greisen",

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AU - Knudsen, Gitte Moos

AU - Greisen, Gorm

PY - 2004

Y1 - 2004

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KW - Laser-Doppler flowmetry

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A newborn rat model for the study of cerebral hemodynamics by near-infrared spectroscopy and laser-Doppler flowmetry in the immature brain

Abstract

Keywords

ASJC Scopus subject areas

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