A new method to assess oxidative stress in ART cycles

Alessandra Di Rosa; Elena Albani; Emanuela Morenghi; Valeria Maria Iommiello; Paolo Emanuele Levi Setti

doi:10.3109/09513590.2015.1110134

A new method to assess oxidative stress in ART cycles

Alessandra Di Rosa, Elena Albani, Emanuela Morenghi, Valeria Maria Iommiello, Paolo Emanuele Levi Setti

Istituto Clinico Humanitas

Research output: Contribution to journal › Article › peer-review

Abstract

The role of oxidative stress (OS) in female reproduction is an understudied area for investigation. The aim of the study was to assess reactive oxygen species (ROS) levels both in follicular fluid (FF) and in serum samples of women undergoing IVF and to relate them to clinical outcomes. A total of 56 women were enrolled. From each patient a sample of serum and FF were collected at oocyte retrieval day and analyzed for OS, by measuring a class of reactive oxygen metabolites (ROMs) (dROMs test) and for biological anti-oxidant potential levels (BAP test). The data analyzed in serum and in FF were related to pregnancy outcomes. There was no significant relationship between d-ROMs and BAP levels in serum or FF GROUP A and B and the clinical parameters of prognosis of women: age, number of oocytes retrieved number of oocytes methaphase II, FSH, fertilization rate and cleavage rate, number of embryos transferred and embryo score. There was a positive association between systemic levels of OS measured in serum GROUP A and pregnancy outcomes (p = 0.007) suggesting that the evaluation of OS in FF needs more investigation about markers in follicular microenvironment in order to predict IVF success.

Original language	English
Pages (from-to)	210-212
Number of pages	3
Journal	Gynecological Endocrinology
Volume	32
Issue number	3
DOIs	https://doi.org/10.3109/09513590.2015.1110134
Publication status	Published - Mar 3 2016

Keywords

Assisted reproductive technology cycles
biological anti-oxidant potential
follicular fluid
oxidative stress
reactive oxygen metabolites
serum

ASJC Scopus subject areas

Endocrinology
Endocrinology, Diabetes and Metabolism
Obstetrics and Gynaecology

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10.3109/09513590.2015.1110134

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title = "A new method to assess oxidative stress in ART cycles",

abstract = "The role of oxidative stress (OS) in female reproduction is an understudied area for investigation. The aim of the study was to assess reactive oxygen species (ROS) levels both in follicular fluid (FF) and in serum samples of women undergoing IVF and to relate them to clinical outcomes. A total of 56 women were enrolled. From each patient a sample of serum and FF were collected at oocyte retrieval day and analyzed for OS, by measuring a class of reactive oxygen metabolites (ROMs) (dROMs test) and for biological anti-oxidant potential levels (BAP test). The data analyzed in serum and in FF were related to pregnancy outcomes. There was no significant relationship between d-ROMs and BAP levels in serum or FF GROUP A and B and the clinical parameters of prognosis of women: age, number of oocytes retrieved number of oocytes methaphase II, FSH, fertilization rate and cleavage rate, number of embryos transferred and embryo score. There was a positive association between systemic levels of OS measured in serum GROUP A and pregnancy outcomes (p = 0.007) suggesting that the evaluation of OS in FF needs more investigation about markers in follicular microenvironment in order to predict IVF success.",

keywords = "Assisted reproductive technology cycles, biological anti-oxidant potential, follicular fluid, oxidative stress, reactive oxygen metabolites, serum",

author = "{Di Rosa}, Alessandra and Elena Albani and Emanuela Morenghi and Iommiello, {Valeria Maria} and {Levi Setti}, {Paolo Emanuele}",

year = "2016",

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doi = "10.3109/09513590.2015.1110134",

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AU - Di Rosa, Alessandra

AU - Albani, Elena

AU - Morenghi, Emanuela

AU - Iommiello, Valeria Maria

AU - Levi Setti, Paolo Emanuele

PY - 2016/3/3

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AB - The role of oxidative stress (OS) in female reproduction is an understudied area for investigation. The aim of the study was to assess reactive oxygen species (ROS) levels both in follicular fluid (FF) and in serum samples of women undergoing IVF and to relate them to clinical outcomes. A total of 56 women were enrolled. From each patient a sample of serum and FF were collected at oocyte retrieval day and analyzed for OS, by measuring a class of reactive oxygen metabolites (ROMs) (dROMs test) and for biological anti-oxidant potential levels (BAP test). The data analyzed in serum and in FF were related to pregnancy outcomes. There was no significant relationship between d-ROMs and BAP levels in serum or FF GROUP A and B and the clinical parameters of prognosis of women: age, number of oocytes retrieved number of oocytes methaphase II, FSH, fertilization rate and cleavage rate, number of embryos transferred and embryo score. There was a positive association between systemic levels of OS measured in serum GROUP A and pregnancy outcomes (p = 0.007) suggesting that the evaluation of OS in FF needs more investigation about markers in follicular microenvironment in order to predict IVF success.

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A new method to assess oxidative stress in ART cycles

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Keywords

ASJC Scopus subject areas

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