A monoclonal antibody specific for prophase phosphorylation of histone deacetylase 1: A readout for early mitotic cells

Chiara V. Segréy; Silvia Senesey; Sara Lopontey; Stefano Santaguida; Paolo Soffientini; Gabriela Grigorean; Mario Cinquanta; Giuseppe Ossolengo; Christian Seiser; Susanna Chiocca

doi:10.1080/19420862.2015.1098795

A monoclonal antibody specific for prophase phosphorylation of histone deacetylase 1: A readout for early mitotic cells

Chiara V. Segréy, Silvia Senesey, Sara Lopontey, Stefano Santaguida, Paolo Soffientini, Gabriela Grigorean, Mario Cinquanta, Giuseppe Ossolengo, Christian Seiser, Susanna Chiocca

Istituto Europeo di Oncologia

Research output: Contribution to journal › Article › peer-review

Abstract

Histone deacetylases (HDACs) are modification enzymes that regulate a plethora of biological processes. HDAC1, a crucial epigenetic modifier, is deregulated in cancer and subjected to a variety of post-translational modifications. Here, we describe the generation of a new monoclonal antibody that specifically recognizes a novel highly dynamic prophase phosphorylation of serine 406-HDAC1, providing a powerful tool for detecting early mitotic cells.

Original language	English
Pages (from-to)	37-42
Number of pages	6
Journal	mAbs
Volume	8
Issue number	1
DOIs	https://doi.org/10.1080/19420862.2015.1098795
Publication status	Published - Jan 1 2016

Keywords

Antibody
Cell cycle
Checkpoint
HDAC1
HDAC2
HDACs
Mitosis
Phosphorylation
Post-translational modifications
Prophase

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1080/19420862.2015.1098795

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abstract = "Histone deacetylases (HDACs) are modification enzymes that regulate a plethora of biological processes. HDAC1, a crucial epigenetic modifier, is deregulated in cancer and subjected to a variety of post-translational modifications. Here, we describe the generation of a new monoclonal antibody that specifically recognizes a novel highly dynamic prophase phosphorylation of serine 406-HDAC1, providing a powerful tool for detecting early mitotic cells.",

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AU - Segréy, Chiara V.

AU - Senesey, Silvia

AU - Lopontey, Sara

AU - Santaguida, Stefano

AU - Soffientini, Paolo

AU - Grigorean, Gabriela

AU - Cinquanta, Mario

AU - Ossolengo, Giuseppe

AU - Seiser, Christian

AU - Chiocca, Susanna

PY - 2016/1/1

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AB - Histone deacetylases (HDACs) are modification enzymes that regulate a plethora of biological processes. HDAC1, a crucial epigenetic modifier, is deregulated in cancer and subjected to a variety of post-translational modifications. Here, we describe the generation of a new monoclonal antibody that specifically recognizes a novel highly dynamic prophase phosphorylation of serine 406-HDAC1, providing a powerful tool for detecting early mitotic cells.

KW - Antibody

KW - Cell cycle

KW - Checkpoint

KW - HDAC1

KW - HDAC2

KW - HDACs

KW - Mitosis

KW - Phosphorylation

KW - Post-translational modifications

KW - Prophase

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A monoclonal antibody specific for prophase phosphorylation of histone deacetylase 1: A readout for early mitotic cells

Abstract

Keywords

ASJC Scopus subject areas

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