A Modified Holder Pasteurization Method for Donor Human Milk: Preliminary Data

Teresa Capriati; Bianca Maria Goffredo; Marta Argentieri; Liliana De Vivo; Paola Bernaschi; Sara Cairoli; Francesca Laureti; Maria Paola Reposi; Daniela Marino; Sabina Benedetti; Antonella Diamanti

doi:10.3390/nu11051139

A Modified Holder Pasteurization Method for Donor Human Milk: Preliminary Data

Teresa Capriati, Bianca Maria Goffredo, Marta Argentieri, Liliana De Vivo, Paola Bernaschi, Sara Cairoli, Francesca Laureti, Maria Paola Reposi, Daniela Marino, Sabina Benedetti, Antonella Diamanti

Ospedale Pediatrico Bambino Gesu

Research output: Contribution to journal › Article › peer-review

Abstract

BACKGROUND: Holder pasteurization (HoP) is the recommended method of pasteurization for donor human milk (DHM). The aim of the present study was to compare nutritional and microbiological impact on DHM of a new technique of pasteurization based on technical changes of HoP.

METHODS: We analyzed milk samples from 25 donors. Each sample, derived from one breast milk expression, was subdivided into three aliquots according to pasteurization: The first was not pasteurized, the second pasteurized by HoP, and the third was pasteurized by modified HoP (MHoP). Each aliquot was assessed as to its microbiological and nutritional profile. Nutritional profile included calcium and triglycerides concentrations detected by spectrophotometry and amino acid levels assessed by high-performance liquid chromatography (HPLC).

RESULTS: Triglycerides were significantly lower in pasteurized, by both methods, than in not pasteurized aliquots, while calcium and amino acids concentration were similar. Microbiological profile did not differ between HoP and MHoP aliquots.

CONCLUSIONS: HoP and MHoP seem to have similar efficacy in preserving some nutritional characteristics of DHM and to confer similar microbiological safety. MHoP is time-saving and potentially costs-effective when compared to HoP, and it is; therefore, potentially of more interest from a practical point of view. Further studies are needed to confirm these findings.

Original language	English
Journal	Nutrients
Volume	11
Issue number	5
DOIs	https://doi.org/10.3390/nu11051139
Publication status	Published - May 22 2019

Access to Document

10.3390/nu11051139

Cite this

@article{f4ac3efc2e2340538b29ac573ed1600f,

title = "A Modified Holder Pasteurization Method for Donor Human Milk: Preliminary Data",

abstract = "BACKGROUND: Holder pasteurization (HoP) is the recommended method of pasteurization for donor human milk (DHM). The aim of the present study was to compare nutritional and microbiological impact on DHM of a new technique of pasteurization based on technical changes of HoP.METHODS: We analyzed milk samples from 25 donors. Each sample, derived from one breast milk expression, was subdivided into three aliquots according to pasteurization: The first was not pasteurized, the second pasteurized by HoP, and the third was pasteurized by modified HoP (MHoP). Each aliquot was assessed as to its microbiological and nutritional profile. Nutritional profile included calcium and triglycerides concentrations detected by spectrophotometry and amino acid levels assessed by high-performance liquid chromatography (HPLC).RESULTS: Triglycerides were significantly lower in pasteurized, by both methods, than in not pasteurized aliquots, while calcium and amino acids concentration were similar. Microbiological profile did not differ between HoP and MHoP aliquots.CONCLUSIONS: HoP and MHoP seem to have similar efficacy in preserving some nutritional characteristics of DHM and to confer similar microbiological safety. MHoP is time-saving and potentially costs-effective when compared to HoP, and it is; therefore, potentially of more interest from a practical point of view. Further studies are needed to confirm these findings.",

author = "Teresa Capriati and Goffredo, {Bianca Maria} and Marta Argentieri and {De Vivo}, Liliana and Paola Bernaschi and Sara Cairoli and Francesca Laureti and Reposi, {Maria Paola} and Daniela Marino and Sabina Benedetti and Antonella Diamanti",

year = "2019",

month = may,

day = "22",

doi = "10.3390/nu11051139",

language = "English",

volume = "11",

journal = "Nutrients",

issn = "2072-6643",

publisher = "NLM (Medline)",

number = "5",

}

TY - JOUR

T1 - A Modified Holder Pasteurization Method for Donor Human Milk

T2 - Preliminary Data

AU - Capriati, Teresa

AU - Goffredo, Bianca Maria

AU - Argentieri, Marta

AU - De Vivo, Liliana

AU - Bernaschi, Paola

AU - Cairoli, Sara

AU - Laureti, Francesca

AU - Reposi, Maria Paola

AU - Marino, Daniela

AU - Benedetti, Sabina

AU - Diamanti, Antonella

PY - 2019/5/22

Y1 - 2019/5/22

N2 - BACKGROUND: Holder pasteurization (HoP) is the recommended method of pasteurization for donor human milk (DHM). The aim of the present study was to compare nutritional and microbiological impact on DHM of a new technique of pasteurization based on technical changes of HoP.METHODS: We analyzed milk samples from 25 donors. Each sample, derived from one breast milk expression, was subdivided into three aliquots according to pasteurization: The first was not pasteurized, the second pasteurized by HoP, and the third was pasteurized by modified HoP (MHoP). Each aliquot was assessed as to its microbiological and nutritional profile. Nutritional profile included calcium and triglycerides concentrations detected by spectrophotometry and amino acid levels assessed by high-performance liquid chromatography (HPLC).RESULTS: Triglycerides were significantly lower in pasteurized, by both methods, than in not pasteurized aliquots, while calcium and amino acids concentration were similar. Microbiological profile did not differ between HoP and MHoP aliquots.CONCLUSIONS: HoP and MHoP seem to have similar efficacy in preserving some nutritional characteristics of DHM and to confer similar microbiological safety. MHoP is time-saving and potentially costs-effective when compared to HoP, and it is; therefore, potentially of more interest from a practical point of view. Further studies are needed to confirm these findings.

AB - BACKGROUND: Holder pasteurization (HoP) is the recommended method of pasteurization for donor human milk (DHM). The aim of the present study was to compare nutritional and microbiological impact on DHM of a new technique of pasteurization based on technical changes of HoP.METHODS: We analyzed milk samples from 25 donors. Each sample, derived from one breast milk expression, was subdivided into three aliquots according to pasteurization: The first was not pasteurized, the second pasteurized by HoP, and the third was pasteurized by modified HoP (MHoP). Each aliquot was assessed as to its microbiological and nutritional profile. Nutritional profile included calcium and triglycerides concentrations detected by spectrophotometry and amino acid levels assessed by high-performance liquid chromatography (HPLC).RESULTS: Triglycerides were significantly lower in pasteurized, by both methods, than in not pasteurized aliquots, while calcium and amino acids concentration were similar. Microbiological profile did not differ between HoP and MHoP aliquots.CONCLUSIONS: HoP and MHoP seem to have similar efficacy in preserving some nutritional characteristics of DHM and to confer similar microbiological safety. MHoP is time-saving and potentially costs-effective when compared to HoP, and it is; therefore, potentially of more interest from a practical point of view. Further studies are needed to confirm these findings.

U2 - 10.3390/nu11051139

DO - 10.3390/nu11051139

M3 - Article

C2 - 31121859

SN - 2072-6643

VL - 11

JO - Nutrients

JF - Nutrients

IS - 5

ER -

A Modified Holder Pasteurization Method for Donor Human Milk: Preliminary Data

Abstract

Access to Document

Fingerprint

Cite this