A human monoclonal autoantibody isolated from a patient with infectious mononucleosis reactive with both self antigens and Epstein-Barr virus nuclear antigen (EBNA)

Carlo Garzelli; Anna Pacciardi; Monica Carmignani; Giulio Conaldi; Fulvio Basolo; Falcone Giuseppe

doi:10.1016/0165-2478(89)90193-4

A human monoclonal autoantibody isolated from a patient with infectious mononucleosis reactive with both self antigens and Epstein-Barr virus nuclear antigen (EBNA)

Carlo Garzelli, Anna Pacciardi, Monica Carmignani, Giulio Conaldi, Fulvio Basolo, Falcone Giuseppe

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Research output: Contribution to journal › Article › peer-review

Abstract

In order to investigate the mechanism(s) by which Epstein-Barr virus (EBV) induces the outcome of autoantibodies during infectious mononucleosis (IM), a human IgM (k) monoclonal antibody to cytoskeletal filaments of epithelial cells has been prepared by EBV transformation of peripheral blood B lymphocytes obtained from a patient with IM. The antibody was also found to react with smooth muscle of frozen sections of human stomach tissue by immunofluorescence, and with the Epstein-Barr nuclear antigen (EBNA) by an enzyme-linked immunosorbent assay. These findings demonstrate at the clonal level the epitope homology between host's cell antigens and EBV-encoded nuclear antigen, which might have relevance in EBV-induced autoimmunity.

Original language	English
Pages (from-to)	211-216
Number of pages	6
Journal	Immunology Letters
Volume	22
Issue number	3
DOIs	https://doi.org/10.1016/0165-2478(89)90193-4
Publication status	Published - 1989

Keywords

EBNA
Epstein-Barr virus
Human monoclonal antibody
Virus-induced autoimmunity

ASJC Scopus subject areas

Immunology
Immunology and Allergy

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10.1016/0165-2478(89)90193-4

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title = "A human monoclonal autoantibody isolated from a patient with infectious mononucleosis reactive with both self antigens and Epstein-Barr virus nuclear antigen (EBNA)",

abstract = "In order to investigate the mechanism(s) by which Epstein-Barr virus (EBV) induces the outcome of autoantibodies during infectious mononucleosis (IM), a human IgM (k) monoclonal antibody to cytoskeletal filaments of epithelial cells has been prepared by EBV transformation of peripheral blood B lymphocytes obtained from a patient with IM. The antibody was also found to react with smooth muscle of frozen sections of human stomach tissue by immunofluorescence, and with the Epstein-Barr nuclear antigen (EBNA) by an enzyme-linked immunosorbent assay. These findings demonstrate at the clonal level the epitope homology between host's cell antigens and EBV-encoded nuclear antigen, which might have relevance in EBV-induced autoimmunity.",

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author = "Carlo Garzelli and Anna Pacciardi and Monica Carmignani and Giulio Conaldi and Fulvio Basolo and Falcone Giuseppe",

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T1 - A human monoclonal autoantibody isolated from a patient with infectious mononucleosis reactive with both self antigens and Epstein-Barr virus nuclear antigen (EBNA)

AU - Garzelli, Carlo

AU - Pacciardi, Anna

AU - Carmignani, Monica

AU - Conaldi, Giulio

AU - Basolo, Fulvio

AU - Giuseppe, Falcone

PY - 1989

Y1 - 1989

N2 - In order to investigate the mechanism(s) by which Epstein-Barr virus (EBV) induces the outcome of autoantibodies during infectious mononucleosis (IM), a human IgM (k) monoclonal antibody to cytoskeletal filaments of epithelial cells has been prepared by EBV transformation of peripheral blood B lymphocytes obtained from a patient with IM. The antibody was also found to react with smooth muscle of frozen sections of human stomach tissue by immunofluorescence, and with the Epstein-Barr nuclear antigen (EBNA) by an enzyme-linked immunosorbent assay. These findings demonstrate at the clonal level the epitope homology between host's cell antigens and EBV-encoded nuclear antigen, which might have relevance in EBV-induced autoimmunity.

AB - In order to investigate the mechanism(s) by which Epstein-Barr virus (EBV) induces the outcome of autoantibodies during infectious mononucleosis (IM), a human IgM (k) monoclonal antibody to cytoskeletal filaments of epithelial cells has been prepared by EBV transformation of peripheral blood B lymphocytes obtained from a patient with IM. The antibody was also found to react with smooth muscle of frozen sections of human stomach tissue by immunofluorescence, and with the Epstein-Barr nuclear antigen (EBNA) by an enzyme-linked immunosorbent assay. These findings demonstrate at the clonal level the epitope homology between host's cell antigens and EBV-encoded nuclear antigen, which might have relevance in EBV-induced autoimmunity.

KW - EBNA

KW - Epstein-Barr virus

KW - Human monoclonal antibody

KW - Virus-induced autoimmunity

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A human monoclonal autoantibody isolated from a patient with infectious mononucleosis reactive with both self antigens and Epstein-Barr virus nuclear antigen (EBNA)

Abstract

Keywords

ASJC Scopus subject areas

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