TY - JOUR
T1 - A fast and simple in situ-PCR method to detect human papilloma virus infection in archival paraffin-embedded tissues
AU - Mancuso, Tommaso
AU - Crovella, Sergio
AU - Casazza, Stefania
AU - Pescarolo, Maria Pia
AU - Clerico, Luana
AU - Russo, Patrizia
AU - Parodi, Silvio
PY - 1997
Y1 - 1997
N2 - We describe a simple and fast method for the detection and localization of low copy numbers of HPV DNA in formalin-fixed and paraffin-embedded archival tissues. We have developed a protocol for direct in situ-PCR in order to demonstrate its convenience in rapid and reproducible assessment of HPV infection in unknown biopsies. The morphological aspect of the tissues has been maintained, despite the multiple steps of fixation, permeabilization and thermal cycling, and positivity has been detected only in virus target cells.
AB - We describe a simple and fast method for the detection and localization of low copy numbers of HPV DNA in formalin-fixed and paraffin-embedded archival tissues. We have developed a protocol for direct in situ-PCR in order to demonstrate its convenience in rapid and reproducible assessment of HPV infection in unknown biopsies. The morphological aspect of the tissues has been maintained, despite the multiple steps of fixation, permeabilization and thermal cycling, and positivity has been detected only in virus target cells.
KW - Archival samples
KW - Human papilloma virus
KW - In situ hybridization
KW - In situ-PCR
KW - Vital infection
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M3 - Article
C2 - 21528242
AN - SCOPUS:0030843822
SN - 1019-6439
VL - 11
SP - 527
EP - 531
JO - International Journal of Oncology
JF - International Journal of Oncology
IS - 3
ER -