8-methoxycaffeine-induced protein-associated DNA breaks in isolated L1210 cell nuclei.

G. Zignaigo, A. Vigani, G. Billi, I. Brega, D. Piccini, L. Poggi, S. Parodi, P. Russo

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8-methoxycaffeine (8-MOC) is a caffeine derivative more potent than its parental compound in inducing chromosomal aberrations. 8-MOC has been postulated to produce chromosomal aberrations by DNA topoisomerase II inhibition. The effect of 8-MOC on nuclear DNA were studied by alkaline elution experiments and compared with those of Ellipticine and Adriamycin (ADR). Like Ellipticine and ADR, 8-MOC induced single strand breaks (SSBs), double strand breaks (DSBs), and DNA-protein cross-links (DPCs) in a bell-shaped manner with respect to drug concentration. As in the case of ADR and Ellipticine, 8-MOC induced equal SSB and DPC frequencies. These results could suggest that 8-MOC induces DNA breaks by interacting with DNA topoisomerase II or with a similar DNA metabolism enzyme.

Original languageEnglish
Pages (from-to)587-594
Number of pages8
JournalBollettino della Societa Italiana di Biologia Sperimentale
Issue number6
Publication statusPublished - Jun 1990

ASJC Scopus subject areas

  • Medicine(all)


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