8-methoxycaffeine-induced protein-associated DNA breaks in isolated L1210 cell nuclei.

G. Zignaigo; A. Vigani; G. Billi; I. Brega; D. Piccini; L. Poggi; S. Parodi; P. Russo

8-methoxycaffeine-induced protein-associated DNA breaks in isolated L1210 cell nuclei.

G. Zignaigo, A. Vigani, G. Billi, I. Brega, D. Piccini, L. Poggi, S. Parodi, P. Russo

Istituto San Raffaele Pisana

Research output: Contribution to journal › Article › peer-review

Abstract

8-methoxycaffeine (8-MOC) is a caffeine derivative more potent than its parental compound in inducing chromosomal aberrations. 8-MOC has been postulated to produce chromosomal aberrations by DNA topoisomerase II inhibition. The effect of 8-MOC on nuclear DNA were studied by alkaline elution experiments and compared with those of Ellipticine and Adriamycin (ADR). Like Ellipticine and ADR, 8-MOC induced single strand breaks (SSBs), double strand breaks (DSBs), and DNA-protein cross-links (DPCs) in a bell-shaped manner with respect to drug concentration. As in the case of ADR and Ellipticine, 8-MOC induced equal SSB and DPC frequencies. These results could suggest that 8-MOC induces DNA breaks by interacting with DNA topoisomerase II or with a similar DNA metabolism enzyme.

Original language	English
Pages (from-to)	587-594
Number of pages	8
Journal	Bollettino della Societa Italiana di Biologia Sperimentale
Volume	66
Issue number	6
Publication status	Published - Jun 1990

ASJC Scopus subject areas

Medicine(all)

Cite this

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title = "8-methoxycaffeine-induced protein-associated DNA breaks in isolated L1210 cell nuclei.",

abstract = "8-methoxycaffeine (8-MOC) is a caffeine derivative more potent than its parental compound in inducing chromosomal aberrations. 8-MOC has been postulated to produce chromosomal aberrations by DNA topoisomerase II inhibition. The effect of 8-MOC on nuclear DNA were studied by alkaline elution experiments and compared with those of Ellipticine and Adriamycin (ADR). Like Ellipticine and ADR, 8-MOC induced single strand breaks (SSBs), double strand breaks (DSBs), and DNA-protein cross-links (DPCs) in a bell-shaped manner with respect to drug concentration. As in the case of ADR and Ellipticine, 8-MOC induced equal SSB and DPC frequencies. These results could suggest that 8-MOC induces DNA breaks by interacting with DNA topoisomerase II or with a similar DNA metabolism enzyme.",

author = "G. Zignaigo and A. Vigani and G. Billi and I. Brega and D. Piccini and L. Poggi and S. Parodi and P. Russo",

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T1 - 8-methoxycaffeine-induced protein-associated DNA breaks in isolated L1210 cell nuclei.

AU - Zignaigo, G.

AU - Vigani, A.

AU - Billi, G.

AU - Brega, I.

AU - Piccini, D.

AU - Poggi, L.

AU - Parodi, S.

AU - Russo, P.

PY - 1990/6

Y1 - 1990/6

N2 - 8-methoxycaffeine (8-MOC) is a caffeine derivative more potent than its parental compound in inducing chromosomal aberrations. 8-MOC has been postulated to produce chromosomal aberrations by DNA topoisomerase II inhibition. The effect of 8-MOC on nuclear DNA were studied by alkaline elution experiments and compared with those of Ellipticine and Adriamycin (ADR). Like Ellipticine and ADR, 8-MOC induced single strand breaks (SSBs), double strand breaks (DSBs), and DNA-protein cross-links (DPCs) in a bell-shaped manner with respect to drug concentration. As in the case of ADR and Ellipticine, 8-MOC induced equal SSB and DPC frequencies. These results could suggest that 8-MOC induces DNA breaks by interacting with DNA topoisomerase II or with a similar DNA metabolism enzyme.

AB - 8-methoxycaffeine (8-MOC) is a caffeine derivative more potent than its parental compound in inducing chromosomal aberrations. 8-MOC has been postulated to produce chromosomal aberrations by DNA topoisomerase II inhibition. The effect of 8-MOC on nuclear DNA were studied by alkaline elution experiments and compared with those of Ellipticine and Adriamycin (ADR). Like Ellipticine and ADR, 8-MOC induced single strand breaks (SSBs), double strand breaks (DSBs), and DNA-protein cross-links (DPCs) in a bell-shaped manner with respect to drug concentration. As in the case of ADR and Ellipticine, 8-MOC induced equal SSB and DPC frequencies. These results could suggest that 8-MOC induces DNA breaks by interacting with DNA topoisomerase II or with a similar DNA metabolism enzyme.

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8-methoxycaffeine-induced protein-associated DNA breaks in isolated L1210 cell nuclei.

Abstract

ASJC Scopus subject areas

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