3p13 region a possible location of a tumor suppressor ghne involved in uveal melanoma

Purpose Uveal melanoma shows monosomy of chromosome 3 as one of the most frequent rearrangements This result leads to the supposition of the involvement of a tumor suppressor gene located on chromosome 3 in the tumorigenesis of this tumor In order to contribute to the identification of the genes responsible for uveal melanoma, we present a case having a structural aberration involving chromosome 3 and 22 Methods A primary cell culture (UM-1) was obtained from a choroida! melanoma, historically characterized as a spindle cell type (dimensions 22 x 15 \10 mm) We utilized F10 medium additioned with 10% Fetal Calf Serum, 1% L- Glutamine. 2% Penicillin-Streptomycin; cytogenetic study was pei formed using both GTG and CBG banding techniques and FISH analysis. Results In ibis cell culture a t(3,22)(p13.:p11) was evidenced, as the only present chromosomal aberration Conclusions. In view of this result, and considering the rearrangement as a possible primary aberration, we would like to stress that the 3p13 breakpoint could be a candidate region for a tumor suppressor gene having an important role in the pathogenesis of this neoplasm.