TY - JOUR
T1 - 3-Methyladenine-DNA-glycosylase and O6-alkyl guanine-DNA-alkyltransferase activities and sensitivity to alkylating agents in human cancer cell lines
AU - Damia, G.
AU - Imperatori, L.
AU - Citti, L.
AU - Mariani, L.
AU - D'Incalci, M.
PY - 1996
Y1 - 1996
N2 - The activities and the expression of 3-methyladenine glycosylase (3-meAde gly) and O6-alkylguanine-DNA-alkyltransferase (O6ATase) were investigated in ten human cancer cell lines. Both 3-meAde gly and O6ATase activities were variable among different cell lines. mRNA levels of the O6ATase gene, appeared to be related to the content of O6ATase in different cell lines, whereas no apparent correlation was found between mRNA of 3-meAde gly and the enzymatic activity. No correlation was found between the activity of the two enzymes and the sensitivity to alkylating agents of different structures such as CC-1065, tallimustine, dimethylsulphate (DMSO), N-methyl-N1-nitro-N-nitrosoguanidine (MNNG), cis-diamminedichloroplatinum (cDDP) and melphalan (L-PAM). The most striking finding of this study is that a correlation exists between the activity of O6ATase and 3-meAde gly in the various cell lines investigated (P <0.01), suggesting a common mechanism of regulation of two DNA repair enzymes.
AB - The activities and the expression of 3-methyladenine glycosylase (3-meAde gly) and O6-alkylguanine-DNA-alkyltransferase (O6ATase) were investigated in ten human cancer cell lines. Both 3-meAde gly and O6ATase activities were variable among different cell lines. mRNA levels of the O6ATase gene, appeared to be related to the content of O6ATase in different cell lines, whereas no apparent correlation was found between mRNA of 3-meAde gly and the enzymatic activity. No correlation was found between the activity of the two enzymes and the sensitivity to alkylating agents of different structures such as CC-1065, tallimustine, dimethylsulphate (DMSO), N-methyl-N1-nitro-N-nitrosoguanidine (MNNG), cis-diamminedichloroplatinum (cDDP) and melphalan (L-PAM). The most striking finding of this study is that a correlation exists between the activity of O6ATase and 3-meAde gly in the various cell lines investigated (P <0.01), suggesting a common mechanism of regulation of two DNA repair enzymes.
KW - 3-methyladenine glycosylase
KW - Alkylating agents
KW - O ATase
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M3 - Article
C2 - 8611396
AN - SCOPUS:0029670361
SN - 0007-0920
VL - 73
SP - 861
EP - 865
JO - British Journal of Cancer
JF - British Journal of Cancer
IS - 7
ER -