23Na-NMR study of cation cotransport in human red blood cells

M. Cacciafesta, V. Marigliano, C. Ferri, G. Piccirillo, A. Scuteri, L. Guidoni, A. M. Luciani, A. Rosi, V. Viti

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Na+-K+ cotransport in human erythrocytes from healthy subjects has been studied by means of 23Na-nuclear magnetic resonance (NMR) in the presence of the anionic paramagnetic shift reagent dysprosium (III) tripolyphosphate [Dy(PPP)2/7-]. The intra- and extracellular 23Na-NMR signals were well separated, giving values of 6 ± 1 mM for internal sodium concentration. Determination of the furosemide-sensitive Na+ efflux, in the presence of ouabain, was obtained by correlating the chemical shift variation of the external signal with changes in external Na+ concentration. For this purpose, calibration curves were generated. The values of the maximum efflux velocity (V(max); 0.29-1 mmol · h-1 · 1 cells-1) measured in 10 healthy adult male subjects were found to be within the range of values obtained by other authors. The NMR method proposed for the study of Na+-K+ cotransport is relatively simple and allows quick evaluation of cotransport V(max) values for a number of samples, being a good candidate for the study of this transport mechanism in the presence of different pathologies.

Original languageEnglish
JournalAmerican Journal of Physiology - Cell Physiology
Issue number5 31-5
Publication statusPublished - 1992


  • normal human erythrocytes
  • ouabain-insensitive sodium effluxes
  • sodium-potassium transport mechanisms

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology
  • Medicine(all)


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